Flow Cytometry is a technique that involves passing suspended cells through a beam of light, typically one or more lasers. As cells pass through the light, the signals they emit are collected and analyzed. These signals provide valuable information about cell characteristics such as viability, size, internal structure, granularity (amount of granules), surface and internal proteins. This allows for a more detailed examination of the cells’ properties. Flow cytometry is widely used in biomedical fields for applications like immunophenotyping, Minimal Residual Disease detection, diagnosis and monitoring of primary immunodeficiencies, stem cell studies, and cell culture research. It is an essential tool for research, diagnosis, and treatment in these areas.